- Describe the radioimmunoassay (RIA) method with respect to the following:
a. What is the purpose of adding a radioactive label to either the antigen or antibody in this type of assay? (0.5 points)
b. Briefly describe the two types of radiation used in the clinical lab. (0.5 points)
- Horseradish peroxidase (HRP), alkaline phosphatase (ALP), and glucose-7-phosphate dehydrogenase are enzymes used in immunochemical methods of detection in the clinical lab.
a. What the name of this immunologic method? (0.25 points)
b. What is the purpose of adding an enzyme to the antigen or antibody? (0.5 points)
c. List the three types of products that result from this method. (0.25 points)
- Describe the radial immunodiffusion method with respect to the following:
a. What type of media (liquid, solid agar etc.) is used? (0.25 points)
b. Which component of the test contains the antibody and which contains the antigen? (0.25 points)
c. Briefly explain (2-3 sentences) the principle upon which the test operates? (0.5 points)
d. What do positive results look like? (0.25 points)
